Order Human SOCS1 Suppressor of Cytokine Signaling 1 EIA Kit 01017054301 at Gentaur SOCS1(Suppressor of Cytokine Signaling 1) Kit
Serum, Plasma, Cell supernatant
Human SOCS1(Suppressor of Cytokine Signaling 1) EIA Kit
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Cytokines and Chemokines are chemotaxis related small proteins between 5 to 20 kDa.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Cell nucleus signaling proteins and molecules are part of a complex system of communication that governs basic cellular activities and coordinates cell actions. The ability of cells to perceive and correctly respond to their microenvironment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in cellular information processing are responsible for diseases such as cancer, autoimmunity, and diabetes. By understanding cell signaling, diseases may be treated effectively and, theoretically, artificial tissues may be created.Kinase, breast cancer tumor, HRAS like - suppressor protein make genetic suppression restoring the phenotype seen prior to the original background in biological pathways.
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human SOCS1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOCS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOCS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOCS1. You can calculate the concentration of Human SOCS1 in the samples by comparing the OD of the samples to the standard curve.